The lay off comes as a major blow for Wales manager Gary Speed just days before he has to prepare his squad to face England and was hoping to select Bale has he had been in excellent form all season.
"Due to a hamstring problem, Gareth Bale has had to withdraw from the Wales squad to face England," a Football Association of Wales statement said.
"He felt tight last week during training with his club.
"After playing 90 minutes on Saturday he felt some muscle tightness. Initially, this was expected to be muscle soreness after playing his first game.
"On joining up with the Welsh squad he was kept off his feet in training for two days to recover.
"As he still felt tight in the warm-up on Tuesday, Gareth was pulled out of the session.
"He did not train at all on Wednesday and was sent for a scan.
"The scan revealed that Gareth Bale picked up his injury last week."